FIG. 4.

Binding of IRF-3 and IRF-7 to IRF binding sites of the IFN and RANTES promoters. (A) Recombinant N-terminal IRF-3 and IRF-7 bind to PRDI-PRDIII and RANTES ISRE probes. An EMSA was performed with the indicated amounts of recombinant protein; the ³²P-labeled probe corresponded to the PRDI-PRDIII region (5′-GAAAACTGAAAGGGAGAAGTGAAAGTG-3′) or the ISRE of the RANTES gene (5′-CTATTTCAGTTTTCTTTTCCGTTTTGTG-3′). (B) Recombinant N-terminal IRF-3 and IRF-7 bind to PRDI and PRDIII probes. An EMSA was performed with the indicated amounts of recombinant protein; the ³²P-labeled probe corresponded to PRDI (5′-GAGAAGTGAAAGTG-3′) or PRDIII (5′-GAAAACTGAAAGGG-3′). (C) Recombinant N-terminal IRF-3 and IRF-7 bind to the PRDI-like and TG sites from the IFNA1, IFNA2, and IFNA14 promoters. An EMSA was performed with the indicated amounts of IRF-3 or IRF-7 and ³²P-labeled probes corresponding to the following PRDI-like sites: IFNA1, 5′-GGAAAGCAAAAACAGAAATGGAAAGTGG-3′; IFNA2, 5′-GAAAGCAAAAAGAGAAGTAGAAAGTAA-3′; and IFNA14, 5′-GGAAAGCCAAAAGAGAAGTAGAAAAAAA-3′.