Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 23;12(12):1100. doi: 10.1038/s41419-021-04390-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — A Real-time PCR was used to evaluate the mRNA level of UBE2C in retinoblastoma cell lines. B The protein levels of UBE2C were detected by western blot in retinoblastoma cell lines. C Knockdown of UBE2C protein in Y79 cells was assessed by western blot. D Knockdown of UBE2C inhibited the proliferation of Y79 cells. **P < 0.01, ***P < 0.001. E A soft agar assay was used to assess the tumour formation ability in vitro. Small colonies were observed and counted under the microscope. Colonies were photographed (left panel) and quantified (right panel). Scale bar: 100 μm. F A colony formation assay was performed to determine the colony formation influence of UBE2C-knockdown Y79 cells. Colonies were photographed (left panel) and quantified (right panel). G Top: Eye weight of the orthotropic xenograft formed by UBE2C-knockdown and Control Y79 cells injected at 30 days after implantation; n = 6, ***P < 0.001. Bottom: H&E staining to evaluate tumour formation. Scale bar: 250 μm. H Representative image of tumour bearing eyeballs removed from the mice at the 30th day.