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. 2021 Nov 23;12(12):1103. doi: 10.1038/s41419-021-04309-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a HOMER3-AS1 expression in SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was measured by qRT-PCR. b Cell proliferation of SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was determined by CCK-8 experiments. c Cell proliferation of SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was determined by EdU incorporation experiments. Scale bars, 100 µm. The red color indicates EdU-positive nucleuses and blue color indicates all cellular nucleuses. Results are shown as the ratio of EdU-positive nucleuses to all cellular nucleuses. d Cell apoptosis of SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was determined by caspase-3 activity assays. e Cell migration of SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was determined by transwell migration assays. Scale bars, 100 µm. f Cell invasion of SK-HEP-1 and SNU-398 cells with HOMER3-AS1 stable overexpression was determined by transwell invasion assays. Scale bars, 100 µm. Results are presented as mean ± SD based on three independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001 by Student’s t-test.