. 2021 Nov 23;11:22799. doi: 10.1038/s41598-021-02065-w

Table 2.

Abbreviations used as follows: Amp^R, ampicillin resistance; Kan^R, kanamycin resistance.

Plasmid	Feature	Marker	Reference
pQE30	E. coli vector for the expression of hexa-His-tagged recombinant proteins	Amp^R	Qiagen
pQE30::isdB NEAT1-NEAT2	pQE30 encoding the NEAT1-NEAT2 domains of IsdB protein from S. aureus SH1000	Amp^R	⁵⁷
pQE30::isdB-NEAT1	pQE30 derivative encoding the NEAT1 domain of IsdB protein from S. aureus SH1000	Amp^R	²⁶
pQE30::isdB NEAT2	pQE30 derivative encoding the NEAT2 domain of IsdB protein from S. aureus SH1000	Amp^R	²⁶
pET28a	E. coli vector for the expression of hexa-His-tagged recombinant proteins	Kan^R	Millipore Sigma
pET28a::vWF-A1	pET28a derivative encoding the A1 domain of the human von Willebrand factor	Kan^R	This study