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. 2021 Nov 23;12:6810. doi: 10.1038/s41467-021-27114-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic of the retrograde tracer CTB488 injection in PFC and CTB555 injection in HP. Digital photomontage showing the CTB555 injection in the HP (left) and CTB488 injection in the PFC (middle) of a P10 CON mouse (total n = 3 mice). Right, digital photomontage displaying CTB555- (red) and CTB488- (green) labeled neurons in LEC of the same mouse. Inset, labeled neurons in LEC shown at higher magnification. The red arrow indicates rhinal fissure. b Left, schematic of the retrograde tracer CTB555 injection in HP. Middle, photographs depicting CTB555-labeled neurons (white dot) in the LEC of a P10 CON and GE mouse. Right, violin plot displaying the number of CTB555-labeled neurons in the LEC of CON (n = 6) and GE (n = 5) mice (one-way ANOVA, p = 0.005, F_{(1, 9)} = 13.44). c Same as b for CTB488 injection in PFC (one-way ANOVA, p = 0.87, F_{(1, 9)} = 0.03). For violin plots, black and red dots correspond to individual animals and the red horizontal lines display the median as well as 25th and 75th percentiles. *p < 0.05.