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. 2021 Nov 23;12(12):1102. doi: 10.1038/s41419-021-04273-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The immunofluorescence detection of MyHC in the loaded (pre-incubated with the OA medium before differentiation) and unloaded cells (pre-incubated with BSA medium). The LDs were stained by BODIPY (green). The nucleus were stained by DAPI. B The fluorescence intensity analysis of MyHC. C Fusion rate analysis of A. D PLIN2 and MyHC levels in loaded and unloaded cells after 4 days of differentiation. E Gray value analysis of western blotting. F The mRNA levels of MyoD, MyoG, MyHC, Myomaker, and Caveolin were detected by qPCR in loaded cells and control cells. *p < 0.05; n.s. not significant. Results are from three technical repeats (N = 3) for a representative of three biological repeats (N = 3).