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. 2021 Nov 23;12(12):1102. doi: 10.1038/s41419-021-04273-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The C2C12 cells were treated with cytochalasin D for 2 h, then the medium was replaced with a fresh medium. The co-localization of PAT-ACTN3-EGFP and ARF1-DsRed was detected. The fluorescence intensity, along with the arrow line, is plotted in the graph below. B The co-localization of LDs and ARF1-EGFP in C2C12 cells was detected. C The rate of actin-filament remodeling in loaded cells with brefeldin A treatment and unloaded cells, bar, 20 μm. D The count of microfilament number per cell. *p < 0.05. Results are from three technical repeats (N = 3) for a representative of three biological repeats (N = 3).