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. 2021 Nov 23;12(12):1102. doi: 10.1038/s41419-021-04273-8

Fig. 7. LDs modulate microfilament remodeling during C2C12 differentiation.

Fig. 7

A The co-localization of PAT-ACTN3-EGFP and microfilaments in myoblast and myotubes. B The subcellular components were isolated from myoblast and myotubes expressing PAT-ACTN3-EGFP or PAT-EGFP. The protein levels of EGFP were detected by Western blotting. C The localization of PAT-ACTN3-EGFP and PAT-EGFP (control) in MyHC+ cells. D The mRNA levels of MyHC and ACTN3 were detected by qPCR in cells expressing PAT-ACTN3-EGFP or PAT-EGFP. E The protein levels of MyHC and ACTN3 were detected by Western blot in cells expressing PAT-ACTN3-EGFP or PAT-EGFP. F The immunofluorescence detection of MyHC in cells expressing PAT-ACTN3-EGFP or PAT-EGFP. G The number of nuclei in MyHC+ cells. GAPDH was utilized as the cytoplasm reference protein, ACTB was utilized as the microfilament reference protein, and ATP1A was utilized as the cytomembrane reference protein. *p < 0.05. Results are from three technical repeats (N = 3) for a representative of three biological repeats (N = 3).