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. 2021 Nov 23;12:6789. doi: 10.1038/s41467-021-27160-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A View of eEF2 and SERBP1 of class I, and B class II. C For comparison on SERBP1 with a canonically bound mRNA, we aligned class I 28S rRNA to 28S rRNA from PDB ID 2Y0G. SERBP1 (purple) occupies the mRNA channel for the ribosome, thus excluding mRNA (dark gray) binding. D The overlay of class I with a recycling factor Hbs1/Pelota-bound ribosome (PDB ID 5LZX) illustrates that recycling factor Pelota and Hbs1-binding is mutually exclusive with bound eEF2/SERBP1. Class I 28S rRNA was aligned to 28S rRNA of the Hbs1/Pelota-bound ribosome.