Table 2. Fold change in miR-146a levels following transfection in primary mixed glial cells.

Forty hours after transfection of vehicle or overexpression vector, total RNA was purified from primary mixed glial cells and their isolated EVs and miR-146a expression levels were assessed.

		miR-146a expression (relative fold change)
Transfection		Intracellular	Extracellular Vesicle
Vector	4 μg/ml	1.0 ± 0.2	1.0 ± 0.0
miR-146a	2 μg/ml	2.1 ± 0.1	53 ± 19
miR-146a	4 μg/ml	2.3 ± 0.4	116 ± 20 *

All data were expressed as mean ± SEM;

^*p<0.05 compared to vector control.