Fig. 4.
(A) Therapeutic effects of HLSC transplantation with various HLSC strains. Each strain of HLSCs were transplanted following 10 weeks of CCl4 treatment. Liver tissues were obtained from rats at 10 weeks (baseline) and at 14 weeks after initiation of CCl4 treatment (post 14w CCl4). Extracellular deposition of collagen fibers was stained with Sirius red. Histopathological changes of liver sections were observed by staining with hematoxylin and eosin (H&E). The presence of the transplanted HLSCs in the liver tissue was identified by immunohistoche-mistry using antibodies against human albumin (hALB). (B) Real-time qPCR analyses of ECM remodeling factors in rat liver specimens according to transplantation of three HLSC strains. Significant increase in MMP2, HGF expression and decrease in TIMP1, TIMP2 expression is seen following transplantation with HLSC7. These changes are not consistently observed in HLSC15 or HLSC26 stains.