Figure 5.
The AαH of Apq12 is important for membrane remodelling. (a) Inactivation of the AαH of Apq12 reduces overexpression toxicity. Growth test of cells containing PGal1-apq12-ah, in comparison to PGal1-APQ12 and vector control cells. Ten-fold serial dilutions of cells were spotted onto glucose (Glu) and galactose/raffinose (Gal/Raf) plates at 30°C. (b–e) Impact of APQ12 and apq12-ah overexpression on (b,c) yeGFP-BRL1 NUP85-tdTomato and (d,e) yeGFP-BRR6 NUP85-tdTomato cells. (c,e)
Quantifications of the maximum GFP intensity of individual cells from (b) and (d) over time. Two independent experiments were performed, and more than 60 cells were analysed per experiment for each time point. Values are given as means ± s.d. t-test ****p < 0.0001. (b,d) Scale bars: 5 µm.