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. 2021 Nov 24;11(11):210250. doi: 10.1098/rsob.210250

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© 2021 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 7. — APQ12 overexpression induces AαH dependent PA accumulation at the NE. (a) Localization of the cytoplasmic PA sensors (Q2-mCherry) in response to P_Gal1-APQ12 and P_Gal1-apq12-ah induction at 0, 1 and 3 h. (b) Line scans of mCherry signal along the indicated lines across the cells in (a). Arrowheads mark the peaks corresponding to the cytoplasmic membrane (c) and NE (N). (c) Quantification of data from (a) showing percentage of cells with nuclear rim localization after 3 h of Gal induction. 100 cells carrying either P_Gal1-APQ12 or P_Gal1-apq12-ah from one representative experiment were analysed for nuclear rim localization of the PA sensor. (d) Localization of the nuclear PA sensors (NLS-Q2-mCherry) in response to P_Gal1-APQ12 (left) and P_Gal1-apq12-ah (right) induction at 0, 1 and 3 h. (e) Line scans of mCherry signal along the indicated lines across the nuclei in (d). Arrowheads mark the peaks corresponding to the NE (N). (f) Quantification of data from (d) showing percentage of cells with nuclear rim localization done as in (c). (a,d) Scale bars: 5 µm.