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. 2021 Nov 24;288(1963):20212168. doi: 10.1098/rspb.2021.2168

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© 2021 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Transcriptomic evidence for deep phylogenetic divergences and expression differences within Chlorokybus. (a) Maximum-likelihood phylogeny based on 529 densely sampled loci, inferred with IQ-TREE under LG + F + I + Γ4 + C60 and support values from 1000 pseudoreplicates of UFBoot2 and SH-aLRT (all branches received 100% support). Branch lengths are in expected substitutions per site. (b,c) Gene expression differences (TMM, trimmed mean of M-values) among four isolates grown simultaneously under the same experimental conditions. Heatmaps correspond to (b) the 9300 annotated proteins in the C. melkonianii genome (no filtering) and (c) the top 200 proteins with the highest expression differences. (Online version in colour.)