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. 2021 Aug 6;373(6555):eabi6226. doi: 10.1126/science.abi6226

Fig. 3. Antigenicity and structures of the B.1.1.7 spike.

Fig. 3.

(A) Binding of ACE2 receptor ectodomain (RBD-directed) and antibodies DH1041 and DH1047 (RBD-directed, neutralizing), DH1050.1 (NTD-directed, neutralizing), and DH1052 (NTD-directed, non-neutralizing) to B.1.1.7 (top) and N501Y (bottom) measured by SPR using single-cycle kinetics. The red lines are the binding sensorgrams; the black lines show fits of the data to a 1:1 Langmuir binding model. The on-rate (kon, M–1 s–1), off-rate (koff, s–1), and binding affinity (KD, nM) for each interaction are indicated. (B to D) Cryo-EM reconstructions of 3-RBD-down states (B), 1-RBD-up states (C), and 1-RBD-up states with disordered RBD (D). The asterisks are placed next to the RBD in the up position. (E) Residue His1118 in the B.1.1.7 spike (PDB 7LWS) and Asp1118 in the D614G spike (PDB 7DKH). (F) Ile716 in the B.1.1.7 spike and Thr716 in the D614G spike. Dashed line shows H-bond with backbone carbonyl of Gln1071.