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. 2021 Nov 24;9:57. doi: 10.1186/s40635-021-00417-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Ex vivo stimulation of 3D microvessels with septic serum before and after therapeutic plasma exchange (TPE). Exemplary 3D reconstruction of the heparan sulfate (HS) layer images of naive endothelial cells in a microfluidic chip (HS in red, DAPI nuclei staining in blue) after perfusion with serum and incubation overnight of a representative septic patient both before and after TPE compared to healthy control serum (pooled out of four control patients) (A) and quantification of the HS positive surface area as box and whisker plots (B)