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mRNA expression level of bone-related genes, including (A) collagen type I (COL I, a fibrotic marker gene), (B) RUNX family transcription factor 2 (RUNX2), (C) osteocalcin (OCN), and (D) alkaline phosphatase (ALP) in bone layer group (B group) and cartilage layer loaded with BMP-2 group (B+BMP-2 group) on days 7 and 14 (n = 3; *P < 0.05).
