Figure 1.

GAB design and binding to CD3+T cells. (A) Schematic representation of the GAB design, showing the extracellular γδTCR domain linked to an anti-CD3 scFv via a flexible linker. (B) Purified GAB was run on SDS-page gel and stained with coomassie brilliant blue protein stain, visualizing the ectoγ-CD3scFv and ectoδ-chain. (C, D) Coating of αβT cells with GAB (10 µg/mL (C) or 90 µg/mL (D)), followed by staining with fluorochrome labeled anti-Vγ9, Vδ2 or pan γδ antibodies. MFI was measured by flow cytometry and represented in histograms. GAB, gamma delta TCR anti-CD3 bispecific molecules