FIGURE 3.

Loss of Rack1 suppresses the proliferation of MB tumor cells. (A) Schematic diagram showing the proliferation of granular neurons was evaluated by BrdU administration and incorporation assay. (B and C) Proliferating granular neurons were immunolabeled with anti‐BrdU and anti‐Ki67 antibodies, respectively. (D and E) Quantitative analysis of proliferating granular neurons shows significantly decreased percentage of BrdU⁺(C) and Ki67⁺(E) cells in rescue mice compared with SHH‐MB tumor mice. **p < 0.01, ***p < 0.001, n ≥ 3. (F) Quantitative RT‐PCR (qRT‐PCR) analysis shows significantly downregulated expression of E2f7, ccnd3, ccne2, cckn2c and cdk4 in rescue mice compared to SHH‐MB tumor mice. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., not significant, n ≥ 3