Figure 1.

EfHTG activities under varied pH, temperature, incubation time or acceptor substrate length.

Activities were tested in vitro with XyG (XET), MLG (MXE) and/or NaOH‐pre‐treated filter paper (CXE) as donor and [³H]XXXGol or polymeric [³H]XyG as acceptor substrate. Enzyme: Pichia‐produced EfHTG.

(a) pH dependence of EfHTG activities in succinate (left) or citrate buffer (right); n = 3; data points are shown as circles.

(b) Temperature dependence of EfHTG activities at pH 5.5 after 24 h incubation; n = 4; data points are shown as circles.

(c) MXE activity measured over 30 days at 6, 23 and 37°C; n = 2.

(d) Comparing CXE activity with [³H]XXXGol or high‐molecular‐weight [³H]XyG as acceptor substrates; the cellulosic product was washed twice in 6 m NaOH: wash 1 was done at 20°C for 16 h and wash 2 at 100°C for 1 h; n = 3; data points are shown as circles.