Figure 2.
Transglucanase activities of EfHTG and EfXTH‐H in the presence of more than one potential donor substrate.
Acceptor substrate: [3H]XXXGol.
(a) Donor substrates: cellulose II (alkali‐pre‐treated filter paper) impregnated with solutions of increasing XyG or MLG concentration and then washed in water before quantification of transglucanase activities (CXE and XET or CXE and MXE). Enzyme: Pichia‐produced EfHTG; n = 3; data points are shown as circles.
(b) Corresponding assays with Pichia‐produced EfXTH‐H acting on cellulose II impregnated with XyG and water‐washed; n = 4; data points are shown as circles.
(c) As (a) but donor substrates: cellulose I and II (untreated and NaOH‐pre‐treated filter papers, respectively) impregnated with different hemicelluloses; n = 4; data points are shown as circles.
KGM, Konjac glucomannan; MLG h. visc. and l. visc, mixed‐linkage β‐glucan with high or low viscosity.