Figure 3.

EfHTG acting on native cell walls.

(a) Equisetum plant, showing zones 1–4, which were used to prepare protein extracts and AIR (scale bar = 20 mm).

(b) Three transglucanase activities exhibited by exogenous enzymes applied to Equisetum cell walls. Enzymes (see key above histograms): EfHTG and co‐extracted EfXTHs extracted from Equisetum zones 1–4 (= Extr. 1–4) or heterologously produced in Pichia; the Pichia‐produced homo‐transglucanase EfXTH‐H served as a control. Donor substrates (photographs below x‐axis; scale bar = 20 mm): Equisetum cell wall material (AIR) from organs of different age (zones 1–4 gave AIR 1–4); acceptor substrate: [³H]XXXGol; mean ± SD; n = 3. XET, MXE and CXE activities are indicated by their ³H‐labelled products: XyG‐[³H]XXXGol, MLG‐[³H]XXXGol and cellulose‐[³H]XXXGol, respectively, formed in the same cell walls at the same time.