Figure 7.

Effect of cellobiose on Equisetum fluviatile shoot elongation and in‐situ localisation of transglucanase actions.

(a) Linear elongation of shoots in the absence of cellobiose (30 days) or in the presence of 10 mm cellobiose for 5 days followed by 10 days without cellobiose and then another 15 days with 10 mm cellobiose; n = 5; data points are shown as green squares/circles (no cellobiose) and blue circles (10 mm cellobiose).

(b) Linear elongation of shoots (as in (a)) exposed to 10 mm glucose for 10 days does not differ from shoots grown without glucose supplementation. n = 5; data points are shown as squares (no glucose) and circles (10 mm glucose).

(c) A 4–5‐week Equisetum fluviatile shoot connected to a rhizome segment, as used for localisation studies (sites for cross‐sectioning in (d) are marked; green shoot top, middle and base, and non‐green rhizome) and for stem growth measurements in (a).

(d) In‐situ localisation of exogenous XXXGol‐SR incorporation by endogenous transglucanases acting for 4 h on endogenous donor substrates in sections of Equisetum shoots and rhizomes in the absence or presence of 10 mm cellobiose. The measured reduction of fluorescence signal intensity of XXXGol‐SR incorporated during 4 h into total cell walls (cellulose + hemicelluloses) of sections (in situ) in the presence of 10 mm cellobiose is shown in % (mean ± SD; n = 4). Scale bar = 250 µm.