Table 1.
Detection limits using the SARS-CoV-2 Analytical Qnostic panel 01. The limit of detection for the SARS-CoV-2 in-house qPCR and the N-gene using the GeneFinder was 50 dC/mL. The RdRp-gene and E-gene using the GeneFinder was 500 dC/mL.
| SARS-CoV-2 Qnostic analytical panel |
In-house SARS-CoV-2 | GeneFinderTM COVID-19 Plus RealAmp kit |
||||
|---|---|---|---|---|---|---|
| Panel number | dC/mL | Log10 dC/mL | E- gene (Ct value) | RdRP- gene (Ct value) | N- gene (Ct value) | E-gene (Ct value) |
| SCVA2AQP01-S01 | 1000000 | 6,00 | 21.87 | 24.22 | 24.17 | 22.27 |
| SCVA2AQP01-S02 | 100000 | 5,00 | 24.66 | 27.09 | 27.11 | 25.46 |
| SCVA2AQP01-S03 | 10000 | 4,00 | 27.87 | 30.07 | 29.74 | 28.77 |
| SCVA2AQP01-S04 | 5000 | 3.7 | 28.52 | 31.11 | 30.62 | 29.73 |
| SCVA2AQP01-S05 | 1000 | 3,00 | 30.57 | 34.39 | 32.8 | 32.88 |
| SCVA2AQP01-S06 | 500 | 2.7 | 31.89 | 43.97 | 34.08 | 35.45 |
| SCVA2AQP01-S07 | 100 | 2,00 | 34.26 | Not detected | 36.4 | Not detected |
| SCVA2AQP01-S08 | 50 | 1.7 | 35.01 | Not detected | 40.14 | Not detected |
| SCVA2AQP01-S09 | Negative | – | Not detected | Not detected | Not detected | Not detected |