Table 3.
Intra- and inter- assay variation of the GeneFinder ™ COVID-19 Plus RealAmp kit using the ELITe InGenius platform. The intra- assay variation of a sample with a high viral load (Ct around 20) was lower compared to a sample with a low viral load (Ct around 31). In contrast, the inter assay variation was comparable for the high and low viral load samples.
| Gene | Sample type | Intra-assay | Inter-assay |
|---|---|---|---|
| Average Ct ± SD (%CV) | Average Ct ± SD (%CV) | ||
| E-gene | high positive | 19.6 ± 0.48 (2.45) | 18.97 ± 1.12 (5.92) |
| low positive | 30.93 ± 1.44 (4.66) | 31.32 ± 0.98 (3.11) | |
| RdRp-gene | high positive | 22.17 ± 0.29 (1.31) | 22.44 ± 0.89 (3.95) |
| low positive | 34.55 ± 1.22 (3.54) | 34.15 ± 1.57 (4.58) | |
| N-gene | high positive | 20.25 ± 0.15 (0.72) | 20.20 ± 1.02 (5.07) |
| low positive | 31.09 ± 1.63 (5.23) | 31.87 ± 0.82 (2.59) |