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Primer sequences for RT–qPCR
| Gene | Forward (5’-3’) | Reverse (3’-5’) |
|---|---|---|
| COL1 | AAGAAGACATCCCTGAAG | AGATACAGATCAAGCATACA |
| BMP2 | CATCACGAAGAAGCCATC | TCATCAGTAGGGACAGAAC |
| Runx2 | AATGCCTCTGCTGTTATG | TTGTGAAGACCGTTATGG |
| ALP | TGATGCTCAGGACAGGAT | GGACCATAAGCGAGTTTCT |
| VEGF | CAGCATAGCAGATGTGAATG | TTCTCCGCTCTGAACAAG |
| GAPDH | CCTGCACCACCAACTGCTTA | GGCCATCCACAGTCTTCTGAG |
