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. 2021 Nov 24;9:188. doi: 10.1186/s40478-021-01288-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Purification and characterization of Frontal Cortex Lewy Bodies (LB) from AD and PDD brains. a Immunohistochemistry images of α-Syn positive LBs (arrows) and LNs (arrowheads) in frontal cortex of postmortem brain samples of 3 of the AD and 3 of the PDD patients used for this study before sequential extraction and purification. Scale bar 50 µm. b Schematic representation of the sequential extraction procedure used to purify pathological α-Syn from frontal cortex of postmortem human brain tissue. c Western blot analysis of purified Sarkosyl-insoluble α-Syn in AD and PDD brains probed with total α-Syn (HuA) and Pα-Syn (81A) antibodies. d ELISA and BCA analysis of purified pathological α-Syn in AD and PDD brains