TABLE 1.
Biosensor-derived kinetic constants for interaction of the recombinant CSD dimer and KAg
| Ligand (RU) | Analyte (M−6) | kona (M−1 s−1) | koffa (s−1) | Kdb (M) | χ2 |
|---|---|---|---|---|---|
| GST-CSDc (500) | hKAg (0.08–1.0) | (3.31 ± 0.042) × 105 | (1.46 ± 0.007) × 10−2 | 4.43 × 10−8 | 9.0 |
| (9.73 ± 0.099) × 103 | (1.17 ± 0.007) × 10−2 | 1.20 × 10−6 | |||
| GST-CSD (150) | hKAg (0.08–1.0) | (1.34 ± 0.032) × 105 | (3.82 ± 0.083) × 10−2 | 5.89 × 10−8 | 3.12 |
| GST-KAg (<50) | hCSD (1.0–10.0) | (1.44 ± 0.067) × 105 | (1.12 ± 0.011) × 10−2 | 7.82 × 10−8 | 0.38 |
a
Standard errors (SE) calculated for globally fitted kinetic constants are shown. Values are means ± SE.
b
Apparent equilibrium dissociation constant calculated from koff/kon.
c
The data were best described by a model in which two independent interaction sites of lower and higher affinities were present (the two sets of values).