FIG. 2.

GR binding to wild-type or GRE mutant MMTV chromatin fragments containing 9 to 10 nucleosomes analyzed by electrophoretic mobility shift in 1% agarose gels. Mobility shift of chromatin on 1% agarose gels. GRE mutants are as described in Table 1. GR concentrations for each chromatin fragment were 0 (lanes 2, 5, 8, and 11), 0.3 (lanes 3, 6, 9, and 12), and 0.5 nM (lanes 4, 7, 10, and 13). Chromatin concentration was 1 ng/μl. Lane 1 contains the DNA fragment alone as marker.