FIG. 3.

Restriction enzyme accessibility in the presence of GR. (A) GR induces a reduction in SacI cleavage [a reduction in fractional cleavage, F(x)] in both naked DNA and reconstituted chromatin. The change in fractional cleavage in the presence of GR (50 nM), ΔF(x), was determined by subtracting the F(x) in the presence of GR from the F(x) in the absence of GR. (B) GR induces a loss in fractional cleavage, F(x), for SacI (1 U) and AlwNI (10 U), but not StuI (10 U) and PstI (2 U). GR concentrations from left to right on all gels were 0, 5, 15, and 50 nM. Chromatin concentration was 1 ng/μl.