Table 2.
Confirmatory RT-qPCR analysis of SARS-CoV-2 DNA in COVID-19−positive samples
| Sample number | N1 | N2 | N4 | ORF1ab | −RevT/qPCR repeat | −RevT/qPCR repeat +DNase |
| 1 | X | X | n/a | n/a | — | — |
| 2 | X | n/a | n/a | — | — | |
| 3 | X | X | n/a | n/a | — | — |
| 4 | X | n/a | n/a | — | — | |
| 5 | X | — | — | |||
| 6 | X | — | — | |||
| 7 | X (1/2) | — | — | |||
| 8 | X (1/2) | — | — | |||
| 9 | X (1/2) | — | — | |||
| 10 | X (2/2) | — | — | |||
| 11 | X | X (2/2) | X | X (2/2) | — | — |
| 12 | X | — | — | |||
| 13 | X | X (1/2) | — | — | ||
| 14 | X (1/2) | X | X (2/2) | N2, N4 | — | |
| 15 | X | N4 | — | |||
| 16 | X (1/2) | X (1/2) | — | — | ||
| 17 | X (1/2) | — | — | |||
| 18 | X (1/2) | — | — | |||
| 19 | X | — | — | |||
| 20 | X | X (2/2) | X | X (2/2) | — | — |
| 21 | X | X (2/2) | X | X (2/2) | — | — |
| 22 | X (1/2) | — | — |
The qPCR for N1, N2, N4, and ORF1ab was repeated for all the samples with amplifications in the −RevT condition. Parentheses are for N2 and ORF1ab display repetition in technical qPCR replicates. An aliquot of −RevT samples was also used for DNase treatment prior to qPCR (last column) to show the DNA nature of possible −RevT signals (X indicates a detected amplification; — indicates no amplification detected; bolded results indicate possible virus genomic insertions; n/a indicates analysis was not performed).