Atomic interfaces between the SipD pentamer and the PrgI needle filament. Ribbon representation of the SipD–PrgI interface. One SipD subunit (yellow) interacts with three PrgI subunits (light green, light cyan, and light pink). The interfaces are highlighted in orange in SipD, and in green, cyan, and pink in PrgI, respectively. The Inset shows the interacting residues (shown as a stick model) targeted for functional analysis.