CgrAlcOx and CdeAlcOx mediated oxidation of some fatty alcohols. (A) Reaction scheme proposed (based on reference 21). (B) Conversion of hexan-1-ol. (C) Conversion of octan-1-ol. Reaction mixtures were incubated for either 15 min or 16 h. All reaction mixtures contained 3 mM substrate and 1 μM AlcOx in phosphate sodium buffer (50 mM, pH 8.0) at 23°C. Reactions varied as follows: no accessory enzyme added, addition of catalase (8 μM final), addition of HRP (12 μM final), addition of both catalase (0.5 μM) and HRP (12 μM final). The key in panel B applies for panel C likewise. Conversion products were analyzed by GC-FID. Error bars show SD values (independent experiments, n = 3).