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Time-dependent loss of G-diketone and GP-1 in vitro when incubated with recombinant LigL, LigN, and LigD, with and without NADH. 0.5 mmol/liter of G-diketone (A) or GP-1 (B) was incubated with each in enzyme with and without 2 mmol/liter NADH. Addition of NADH initiated the reaction. Samples were incubated in the dark at 30°C. Concentrations of G-diketone and GP-1 was measured using HPLC-MS.
