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. 2021 Nov 24;87(24):e01742-21. doi: 10.1128/AEM.01742-21

TABLE 3.

Primers for confirming lig deletion mutants (29)

Name Forward primer Reverse primer Target
01025 TCAGGTCCACCAGTTCGCCATC GTCTCTATCGCGTTGACCGACTGG ligD
03960 ACAAGAACTTCGGCCTCTATCGTGAC GTGAAGCTCGACGTGACCAATCG ligO
03965 CGCGAACTTGGTGGTATTGTAGATGC CGAAAAGGCGCGAGTGATCTTCTTC ligN
09390 GCTATGCCGAATTTGCCCTGAC CTGTCGGGATATGCCATCTACATCTGG ligL