FIG 1.
Response of AN2343 to external menadione. (A) Q-RT-PCR analysis of AN2343 induced by menadione. After preculture for 16 h, the WT strain was exposed to 0.8 mM menadione (MEN) for 3 h. The relative mRNA levels were normalized to that of actA. Error bars represent the standard deviations (SD) of three independent experiments. (B) Deleterious effect of AnNTR on protection against menadione. After incubation of A. nidulans conidiospores on agar plates containing various concentrations of menadione at 37°C for 48 h, the colony morphology was examined to determine the sensitivities to menadione. (C) Recovery of menadione sensitivity by the expression of AnNTR-GFP in ΔAN2343 and the subcellular localization of AnNTR-GFP. The AnNTR::GFP fusion protein was expressed using the native promoter and terminator of AN2343. Conidiospores (1 × 105) from the WT, ΔAN2343, and ΔAN2343-com (ΔAN2343 complemented with AnNTR-GFP) strains were cultivated on minimal medium agar plates supplemented with 125 μM MEN at 37°C for 48 h. The fluorescence from strains cultivated in liquid MM without menadione for 16 h was observed by using confocal laser scanning microscopy.