Transcription levels of AN2343 in response to nitro-compounds and amino acids. After preculture for 16 h in minimal medium, the WT strain was incubated in the presence of a series of nitro-compounds (0.3 mM) or amino acids (5 mM) for 3 h. The relative mRNA expression levels are shown compared to the untreated strain and normalized to actA. E-PNB, ethyl p-nitrobenzoate; TNT, 2,4,6-trinitrotoluene; 4-NQO, 4-nitroquinoline N-oxide; MTZ, metronidazole; PLU, plumbagin; L-Phe, l-phenylalanine; L-Tyr, l-tyrosine; L-Glu, l-glutamate; L-Ala, l-alanine. The data show the means ± the SD of three independent experiments.