TABLE 1.
Activity of recombinant AnNTR with different substrates
| Substrate | Mean activity (ΔA590 min−1 mg protein−1) ± SDa |
|---|---|
| Nitroaromatics | |
| 2-Nitrotoluene | 0.13 ± 0.09 |
| 4-Nitrotoluene | 0.09 ± 0.08 |
| 1,3-Dinitrobenzene | 0.15 ± 0.09 |
| 2-Chloronitrobenzene | 0.09 ± 0.08 |
| 4-Chloronitrobenzene | 0.01 ± 0.00 |
| 2-Nitrobenzaldehyde | 1.21 ± 0.09 |
| 3-Nitrobenzaldehyde | 0.28 ± 0.08 |
| 4-Nitrobenzaldehyde | 1.18 ± 0.09 |
| 2-Nitrobenzoate | 0.21 ± 0.09 |
| 3-Nitrobenzoate | 0.37 ± 0.08 |
| 4-Nitrobenzoate | 0.24 ± 0.08 |
| Ethyl p-nitrobenzoate | 0.09 ± 0.05 |
| 4-Nitrophenol | 0.27 ± 0.08 |
| 2,4,6-Trinitrotoluene | 0.51 ± 0.03 |
| Chloramphenicol | 0.44 ± 0.22 |
| Nitroheterocyclic derivatives | |
| Nitrofurantoin | 2.20 ± 0.23 |
| Metronidazole | 1.73 ± 0.22 |
| 4-Nitroquinoline N-oxide | 3.09 ± 0.22 |
| Quinones | |
| Menadione | 0.64 ± 0.22 |
| Plumbagin | 7.68 ± 0.35 |
a
The activities of recombinant AnNTR with various nitro- and non-nitro compounds (50 μM) as the substrates are shown. Functional assays were performed with MTT as a chromogenic electron acceptor and NADPH as an electron donor. The activity was measured as the increase of MTT formazan (590 nm) in the first 0- to 60-s reaction at 25°C. Mean values are given for three replicates after subtraction of substrate blanks.