FIG. 3.
Effects of IGFBP-3 and E7 on apoptosis rate in PC-3 cells. PC-3 cells were transfected with expression vectors for IGFBP-3 and/or E7, as indicated. (A) The rate of apoptosis was determined by flow cytometry. Cells with sub-G1 DNA content were considered to be apoptotic. Values are the means ± standard deviation of three independent experiments (P < 0.005). wt, wild type. (B) The levels of IGFBP-3 in both cellular lysates and conditioned medium and levels of E7 proteins were determined by immunoblotting. M2 pruvate kinase (M2-PK) served as a loading control.