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BAY regulates the function of microglia after TBI. (A) Microglial density was measured in mouse brain tissues following TBI using Iba-1 immunofluorescence staining. Scale bar, 50 µm (Iba-1, red; DAPI, blue). Microglial migration and chemotaxis were examined using. (B) Transwell assay and (C) agarose spot test. Scale bar, 50 µm. **P<0.01, ****P<0.0001. BAY, BAY61-3606; TBI, traumatic brain injury; Iba-1, ionized calcium binding adaptor molecule 1; LPS, lipopolysaccharide.
