Table 1.

Multiple cancer cell lines lacking p53 require MDM2 and MDMX to maintain the cell cycle and survival

Cell line	p53 status	Response to siMDM2	Response to MEL23
H1299	Null	Arrest	Arrest and death
MDA-MB-231 CRISPR p53KO	Null	Arrest	Arrest and death
MCF7 CRISPR p53KO	Null	Arrest	Arrest and death
A549 CRISPR p53KO	Null	Arrest	No effect
H1299 tet-R175H	R175H	Arrest	Arrest and death
H1299 tet-R248W	R248W	Arrest	Arrest and death
MDA-MB-231	R280K	Arrest	Arrest and death
SKBR3	R175H	Arrest	Arrest and death

Cell-cycle arrest in response to MDM2 knockdown or MEL23 treatment in different cancer cells lacking p53 expression or expressing different p53 point mutants. The cell cycle was measured as in Figs. 1, 2, and 3. “Arrest” indicates reduction in the percentage of cells in S phase. “Death” was measured by the percentage of cells in sub-G1. All data represent at least three biological replicates.