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. 2021 Nov 12;19(11):e3001450. doi: 10.1371/journal.pbio.3001450

Fig 7. Nfia and Nfib are required for the efficient generation of late-born Neurod2 neurons (see also S14 Fig).

Fig 7

(A) Analysis of Nfia and Nfib ChIP-Seq data from the mouse cerebellum [77] confirms binding of Nfia and Nfib to genomic regions in the vicinity of the Neurod2 gene. (B) Analysis of ChIP-seq data from the ENCODE project [78] reveals accumulation of H3K27ac at the same sites between e11.5 and e13.5 in the different regions of the nervous system. (C) Neurod2 (top) and Zfhx3 (bottom) intensity histograms in control (left) and Nfia; Nfib double mutant (right) neurons (red) and progenitors (blue) at D11 in ventral conditions. Shading indicates the applied thresholds above which cells were counted as Neurod2 or Zfhx3-positive. (D) Percentage of Neurod2 (top) and Zfhx3-positive neurons (bottom) at D11 in control and Nfia; Nfib double mutants differentiated in ventral conditions (n = 6 for control and n = 3 for Nfia; Nfib double mutants). Significance was assessed by unpaired t-test with Welch’s correction. Underlying flow cytometry data are provided in S3 Data. H3K27ac, Histone-3-Lysine-27-acetylation.