Fig. 2. The G454E variant restores light-dependent xanthan production phenotype in vivo.

Wild-type (Xcc), Xccbphp, pXccBphP, and pG454E strains were grown to stationary phase in 20 ml of PYM media supplemented with 2% glucose under darkness or far-red (733 nm) light conditions. Xanthan was purified through ethanol precipitation of cell-free supernatants. Cell pellets and precipitated extracellular xanthan were dried and weighed. Data are represented by mean values ± SEM from nine independent experiments (three replicates per experiment). Data were analyzed using the Kruskal-Wallis test. Different letters indicate significant differences between groups (P < 0.05).