Figure 4.

The activity of 0 (medium only), 10, and 20 μmol/L apigenin (AP) pre-dissolved in DMSO (Free AP) or nanoencapsulated in WPI (En-AP), or WPI processed under the same pH cycle treatment (pH-treat-WPI), before and after simulated gastric and intestinal digestions, against the proliferation of (A) HCT-116 and (B) HT-29 colon cancer cells after 72-h incubation at 37 °C. The En-AP was prepared with 10.0 mg/mL WPI and 2 mg/mL AP using the pH-cycle. The pH-treat-WPI was prepared in the equivalent amount of that in En-AP. Error bars are SD (n = 3). Columns with different letters represented significant differences (p < 0.05).