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Uptake of apigenin (AP) by (A) HCT-116 and (B) HT-29 colon cancer cells after treating with 5, 10, and 20 μmol/L of AP pre-dissolved in DMSO (Free AP) or nanoencapsulated in WPI (En-AP) for 24 h at 37 °C. The En-AP was prepared with 10.0 mg/mL WPI and 2 mg/mL AP using the pH-cycle. Error bars are SD (n = 3). Different letters above columns indicate significant difference between Free AP and En-AP at the same AP concentration (p < 0.05).
