Figure 5.

Effect of young and old human serum on proliferation, apoptosis and differentiation of human hippocampal progenitor cells. (A) Ki67 - proliferating cells; (B and C) Cleaved Caspase 3 (CC3) - apoptotic cell death; (D) DCX - neuroblasts; (E) Map2 - immature neurons following 9 days of culture with young (n=30, 15 females, mean age of 27.1 years) or old (n=35, 20 females, mean age of 77.6 years) human serum at a concentration of 1%, 3 technical replicates per sample. All presented as % of total number of cells. Unpaired student and Mann Whitney t-tests used as appropriate. * P < 0.05. Error bars = SEM.