Table 2.

Table showing key genes identified in this study in relation to aging literature.

Gene	Current Study	Existing Literature
PARP1	Decrease in response to old serum (6 days timepoint, qPCR)	PARP1 is overactivated in aging and age-related disease, inhibition of PARP1 was shown to be beneficial in delaying progression [65-67].
UCHL	Increase in response to old serum (6 days timepoint, qPCR)	An increase in UCHL was shown in aging mice. In addition, plasma UCHL levels showed a positive correlation with Parkinson’s disease severity in a recent case-control study [68, 69].
TMEM149	Increase in response to old serum (6-hour timepoint, identified via microarray, validated by qPCR)	Role of TMEM149 in aging remains largely unexplored. However, a recent longitudinal multiomics profiling study in healthy individuals showed a positive correlation between TMEM149 and age [70].
RFN126	Decrease in response to old serum (6-hour timepoint, identified via microarray, validated by qPCR)	RNF126 is required for Bag6-dependent ubiquitination and has been shown to initiate degradation of mislocalised and misfolded proteins. This suggests a decrease in RNF126 may be associted to an accumulation of mislocalised and misfolded proteins [71, 72].
ENDOG	Increase in response to old serum (6-day timepoint, identified via microarray, validated by qPCR)	Higher levels of EndoG were found in whole brain lystaes of 34-week-old mice when compared with 5-week-old mice. In addition, nuclear levels of ENGOG were 153% higher in the aged rat plantaris at 24 months, when compared to younger counterparts aged 6 months [73, 74].