Figure 4.

dTrpA1 expression in lPN4 suppressed the expression of post-tapping behaviors in males. A, Frequency of tapping until the first unilateral wing extension at 31°C in the dTrpA1-expressing males (6107/Trp) and controls (+/Trp). B, Ratio of male flies that showed the first unilateral wing extension by the fourth tapping of a female at 31°C. C, Frequency of unilateral wing extension observed in 10 min at 31°C. D, Frequency of copulation attempts in 10 min at 31°C. E, Movement activities in 1 min at 31°C. F–H, The frequencies of tapping until the first unilateral wing extension (F), unilateral wing extension (G), and copulation attempts (H) were not statistically different between controls and those flies expressing dTrpA1 in lPN4 at 25°C. Statistical analyses were performed using a generalized linear mixed model (A, F), two-sided Fisher's exact test (B), two-tailed Mann–Whitney test (C, D), and unpaired two-tailed t test (E, G, H). Numbers within bars and above error bars represent the numbers of animals observed. Error bars indicate the SEM.