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. 2021 Oct 29;10:e72841. doi: 10.7554/eLife.72841

Figure 5. Loss of Tomosyn does not affect AZ number, NMJ area or SV abundance.

Figure 5.

(A) Representative confocal images of immunohistochemistry against BRP (nc82) and neuronal membranes (anti-HRP). (B) Quantification of AZ number per muscle 4 NMJ for Ib, Is and both (control, Ib: 296.0, 288.2 ± 10.71, n = 17; control, Is: 115.5, 115.8 ± 14.97, n = 12; tomosynNA1, Ib: 246.0, 249.7 ± 7.824, n = 17; tomosynNA1, Is: 126.0, 134.1 ± 12.52, n = 17; control, total: 378.0, 374.8 ± 25.07, n = 14; tomosynNA1, total: 366.0, 383.9 ± 14.74, n = 17; p = 0.0001; ≥ 7 larvae per group). (C) Quantification of average BRP abundance per AZ per muscle 4 NMJ, measured as average of maximum pixel intensity of each BRP puncta in arbitrary fluorescence intensity units (control, Ib: 19911, 20722 ± 1210,, n = 17; control, Is: 20682, 21733 ± 1448,, n = 12; tomosynNA1, Ib: 21430, 20681 ± 895.7, n = 17; tomosynNA1, Is: 22275, 22172 ± 1372,, n = 17; p = 0.7654; ≥ 7 larvae per group). (D) Quantification of average bouton size (um2) per muscle 4 NMJ measured as the HRP positive area for each bouton along the arbor (control, Ib: 7.489, 12.16 ± 1.013, n = 195; control, Is: 4.342, 4.974 ± 0.2488, n = 140; tomosynNA1, Ib: 7.508, 8.953 ± 0.3671, n = 241; tomosynNA1, Is: 4.873, 5.413 ± 0.1777, n = 229; p = 0.7654; ≥ 7 larvae per group). (E) Quantification of muscle 4 NMJ area (um2) measured as HRP positive area (control, Ib: 176.9, 176.0 ± 8.056, n = 17; control, Is: 69.76, 88.9 ± 12.89, n = 12; tomosynNA1, Ib: 164.5, 164.7 ± 7.527, n = 17; tomosynNA1, Is: 84.32, 99.69 ± 11.80, n = 17; p < 0.0001; ≥ 7 larvae per group). (F) Quantification of muscle four bouton number per Ib motoneuron (control: 17.50, 17.19 ± 1.030, n = 16; tomosynNA1: 20, 20.19 ± 0.7025, n = 16; p = 0.0225 ≥ 7 larvae per group). (G) Representative NMJs stained with Syt1 antisera. (H) Quantification of Syt1 expression (sum of arbitrary fluorescence units) per Ib motoneuron (control: 3.913*10^8, 4.083*10^8 ± 0.949* 10^8, n = 16; tomosynNA1: 3.3900* 10^8, 3.6713*10^8 ± 0.1297*10^8, n = 16; p = 0.0892 ≥ 7 larvae per group). Complete data found in Figure 5—source data 1.

Figure 5—source data 1. Source data for Figure 5.