Figure 1.

Effect of aimR and aimP mutations on phage titer

(A) 168 Δ6 strains lysogenic for phage SPβ WT, ΔaimR, and ΔaimP were MC induced (0.5 μg/mL), and the number of resulting phages were quantified by titering using 168 Δ6 as the recipient strain. The results are represented as the plaque-forming units (PFUs) mL⁻¹. The means and SDs are presented (n = 4). An ordinary one-way ANOVA of transformed data was performed to compare mean differences between SPβ WT, ΔaimR, and ΔaimP titers. Adjusted p values were as follows: SPβ ΔaimR ^∗∗∗∗p ≤ 0.0001; SPβ ΔaimP ^∗p = 0.0115.

(B) 168 Δ6 strains lysogenic for phages phi3T WT, ΔaimR, and ΔaimP were MC induced (0.5 μg/mL), and the number of resulting phages were quantified by titering using 168 Δ6 as the recipient strain. The results are represented as PFUs/mL⁻¹. The means and SDs are presented (n = 3). An ordinary one-way ANOVA of transformed data was performed to compare mean differences between SPβ WT, ΔaimR, and ΔaimP titers. Adjusted p values were as follows: SPβ ΔaimR ^∗∗∗∗p ≤ 0.0001; SPβ ΔaimP ^∗∗p = 0.0058.

(C) Strain 168 lysogenic for phages SPβ WT, ΔaimR, and ΔaimP were MC induced (0.5 μg/mL), and the number of resulting phages was quantified by titering using 168 Δ6 as the recipient strain. The results are represented as PFUs/mL⁻¹. The means and SDs are presented (n = 3). An ordinary one-way ANOVA of transformed data was performed to compare mean differences between SPβ WT, ΔaimR, and ΔaimP titers. Adjusted p values were as follows: SPβ ΔaimR ^∗∗∗∗p ≤ 0.0001; SPβ ΔaimP ns, not significant.